Tag

Part:BBa_K3581002:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


6x His Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of TEV protease cut site in front of the tag to facilitate purification.


Source

Original sequence from iGEM13 BGU Israel.

References

1. England, N. (n.d.). TEV Protease | NEB. Retrieved October 13, 2020, from https://international.neb.com/products/p8112-tev-protease#Product%20Information 2. Wikipedia. (2020, October 14). TEV Protease. Wikipedia. Retrieved October 13, 2020, from https://en.wikipedia.org/wiki/TEV_protease